GRK 1721

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Gregor Witte - Hybrid Methods Approaches in DNA Damage

Methods and Experiences

Due to the increasing complexity of biological problems I am trying to use a combination of biophysical/biochemical techniques - like e.g. the combination of X-ray crystallography, Modeling and SAXS. The application of these hybrid method approaches will provide a more detailed picture and thus leads to a better understanding of the complex problems.

Biophysical Methods and Experiences

  • X-ray crystallography (MAD/SAD/MR, high-throuput crystallization trials, refinements)
  • (cryo)-Electron Microscopy
  • SAXS (SECSAXS, batch, ab initio modeling, flexible modeling, rigid body docking,...)
  • Absorption/Fluorescence Spectroscopy (FRET, Anisotropy, Quenching)
  • Fast Kinetics
  • Analytical Ultracentrifugation
  • NMR (collarboration with Rasmus Linser / Elias Akoury )
  • Surface Plasmon Resonance (kinetcs, equillibrium)
  • Isothermal Titration Calorimetry
  • Static/Dynamic Light Scattering (Determination of molecular weight in solution by SEC-RALS, Rh-distribution by DLS)
  • Differential Scanning fluorimetry (Thermal shift analysis, Thermofluor using SYPRO-orange based assays or intrinsic Trp-fluorescence)
  • Chromatography methods (HPLC, SEC, RPC, protein purification steps)
  • Standard biochemical assays 
  • Mass-spectrometry
  • Computing and IT