Gregor Witte - Hybrid Methods Approaches in DNA Damage
Methods and Experiences
Due to the increasing complexity of biological problems I am trying to use a combination of biophysical/biochemical techniques - like e.g. the combination of X-ray crystallography, Modeling and SAXS. The application of these hybrid method approaches will provide a more detailed picture and thus leads to a better understanding of the complex problems.
Biophysical Methods and Experiences
- X-ray crystallography (MAD/SAD/MR, high-throuput crystallization trials, refinements)
- (cryo)-Electron Microscopy
- SAXS (SECSAXS, batch, ab initio modeling, flexible modeling, rigid body docking,...)
- Absorption/Fluorescence Spectroscopy (FRET, Anisotropy, Quenching)
- Fast Kinetics
- Analytical Ultracentrifugation
- NMR (collarboration with Rasmus Linser / Elias Akoury )
- Surface Plasmon Resonance (kinetcs, equillibrium)
- Isothermal Titration Calorimetry
- Static/Dynamic Light Scattering (Determination of molecular weight in solution by SEC-RALS, Rh-distribution by DLS)
- Differential Scanning fluorimetry (Thermal shift analysis, Thermofluor using SYPRO-orange based assays or intrinsic Trp-fluorescence)
- Chromatography methods (HPLC, SEC, RPC, protein purification steps)
- Standard biochemical assays
- Mass-spectrometry
- Computing and IT