Gregor Witte - Hybrid Methods Approaches in DNA Damage

Methods and Experiences

Due to the increasing complexity of biological problems I am trying to use a combination of biophysical/biochemical techniques - like e.g. the combination of X-ray crystallography, Modeling and SAXS. The application of these hybrid method approaches will provide a more detailed picture and thus leads to a better understanding of the complex problems.

Biophysical Methods and Experiences

• X-ray crystallography (MAD/SAD/MR, high-throuput crystallization trials, refinements)
• (cryo)-Electron Microscopy
• SAXS (SECSAXS, batch, ab initio modeling, flexible modeling, rigid body docking,...)
• Absorption/Fluorescence Spectroscopy (FRET, Anisotropy, Quenching)
• Fast Kinetics
• Analytical Ultracentrifugation
• NMR (collaboration with Rasmus Linser)
• Surface Plasmon Resonance (kinetcs, equillibrium)
• Isothermal Titration Calorimetry
• Static/Dynamic Light Scattering (Determination of molecular weight in solution by SEC-RALS, Rh-distribution by DLS)
• Differential Scanning fluorimetry (Thermal shift analysis, Thermofluor using SYPRO-orange based assays or intrinsic Trp-fluorescence)
• Chromatography methods (HPLC, SEC, RPC, protein purification steps)
• Standard biochemical assays 
• Mass-spectrometry
• Computing and IT