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Gregor Witte - Hybrid Methods Approaches in DNA Damage

2022 | 20212020 | 2019  | 2018 |  2017   |   2016   |   2015   |   2014   |   2013   |   2012   |   2011   |   2010 and before

 2022  

Chemical synthesis of the fluorescent, cyclic dinucleotides cthGAMP
Veth S, Fuchs A, Özdemir D, Dialer C, Drexler DJ, Knechtel F, Witte G, Hopfner KP, Carell T, and Ploetz E
ChemBioChem, 2022;  doi: 10.1002/cbic.202200005

 2021  

BusR senses bipartite DNA binding motifs by a unique molecular ruler architecture
Bandera AM, Bartho J, Lammens K, Drexler DJ, Kleinschwärzer J, Hopfner KP, and Witte G*
Nucleic Acids Research, 2021;, gkab736  doi: 10.1093/nar/gkab736

Assessment of Diadenylate Cyclase and c-di-AMP-phosphodiesterase Activities Using Thin-layer and Ion Exchange Chromatography.
Latoscha, A., Drexler, D. J., Witte, G. and Tschowri, N.
Bio-protocol 11(1): e3870. DOI: 10.21769/BioProtoc.3870

2020

C-Terminal Motifs of the MTW1 Complex Cooperatively Stabilize Outer Kinetochore Assembly in Budding Yeast.
Ghodgaonkar-Steger M, Potocnjak M, Zimniak T, Fischböck-Halwachs J, Solis-Mezarino V, Singh S, Speljko T, Hagemann G, Drexler DJ, Witte G, Herzog F.
Cell Rep.
2020 Sep 29;32(13):108190. doi: 10.1016/j.celrep.2020.108190.

Structural basis for sequestration and autoinhibition of cGAS by chromatin.
Michalski S, de Oliveira Mann CC, Stafford C, Witte G, Bartho J, Lammens K, Hornung V, Hopfner KP.
Nature. 2020 Sep 10. doi: 10.1038/s41586-020-2748-0.

C-di-AMP hydrolysis by the phosphodiesterase AtaC promotes differentiation of multicellular bacteria
Latoscha A and Drexler DJ, Al-Bassam MM, Bandera AM, Kaever V, Findlay KC, Witte G* and Tschowri N*
PNAS March 18, 2020. https://doi.org/10.1073/pnas.1917080117

2019

A Click-Chemistry Linked 2'3'-cGAMP Analog.
Dialer CR, Stazzoni S, Drexler DJ, Müller FM, Veth S, Pichler A, Okamura H, Witte G, Hopfner KP, Carell T.
Chemistry. 2019 Feb 6;25(8):2089-2095

2018

Reproducibility in crisis: Sample quality and the importance of early and ongoing analysis.
Science/AAAS webinar 14.03.2018 @  www.sciencemag.org

Qualitätskontrolle von Proteinen durch thermische Entfaltungsprofile
Breitsprecher D, Kern B, Gupta AJ, Knoll K, Witte G
Biospektrum. 1/2018 pg 60-62

2017

Structural and Biophysical Analysis of the Soluble DHH/DHHA1-Type Phosphodiesterase TM1595 from Thermotoga maritima
Drexler DJ, Müller M, Rojas-Cordova CA,Bandera AM, Witte G
Structure. 2017 Oct 24. pii: S0969-2126(17)30329-5.

Cyclic Dinucleotides in the Scope of the Mammalian Immune System.
Mankan AK, Müller M, Witte G, Hornung V.
Handb Exp Pharmacol. 2017 Feb 9

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2016

Atomic Structure and Biochemical Characterization of an RNA Endonuclease in the N Terminus of Andes Virus L Protein
Fernández-García Y, Reguera J, Busch C, Witte G, Sánchez-Ramos O, Betzel C, Cusack S, Günther S, Sophia Reindl
PLOS Pathog DOI:10.1371/journal.ppat.1005635 June 14, 2016

Structural and biochemical characterization of the cell fate determining nucleotidyltransferase fold protein MAB21L1.
de Oliveira Mann CC, Kiefersauer R, Witte G*, Hopfner KP.
Sci Rep. 2016 Jun 8;6:27498. doi: 10.1038/srep27498

cGAS Senses Human Cytomegalovirus and Induces Type I Interferon Responses in Human Monocyte-Derived Cells.
Paijo J, Döring M, Spanier J, Grabski E, Nooruzzaman M, Schmidt T, Witte G, Messerle M, Hornung V, Kaever V, Kalinke U.
PLoS Pathog. 2016 Apr 8;12(4):e1005546.

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2015

Protein translocation channel of mitochondrial inner membrane and matrix-exposed import motor communicate via two-domain coupling protein.
Banerjee R, Gladkova C, Mapa K, Witte G, Mokranjac D.
Elife. 2015 Dec 29;4. pii: e11897. doi: 10.7554/eLife.11897.

Structural analysis of the di-adenylate cyclase reaction of DNA-integrity scanning protein A (DisA) and its inhibition by 3'-dATP.
Müller M, Deimling T, Hopfner KP, Witte G.
Biochem J. 2015 May 27. [Epub ahead of print]

c-di-AMP recognition by Styphylococcus aureus PstA
Müller M., Hopfner KP and Witte G
FEBS Lett. 2015 Jan 2;589(1):45-51. doi: 10.1016/j.febslet.2014.11.022. Epub 2014 Nov 28.

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2014

Crystal and solution structure of the human RIG-I SF2 domain
T. Deimling, S. Cui, K. Lammens, K.-P. Hopfner and G. Witte
Acta Cryst. (2014). F70, 1027-1031

The inverse BAR-domain protein IBARa drives membrane remodelling to control osmoregulation, phagocytosis and cytokinesis
Linkner J, Witte G*, Zhao H, Junemann A, Nordholz B, Runge-Wollmann P, Lappalainen P, Faix J
J Cell Sci. 2014 Jan 24. [Epub ahead of print]

Optimized ratiometric calcium sensors for functional in vivo imaging of neurons and T lymphocytes.
Thestrup T, Litzlbauer J, Bartholomäus I, Mues M, Russo L, Dana H, Kovalchuk Y, Liang Y, Kalamakis G, Laukat Y, Becker S, Witte G, Geiger A, Allen T, Rome LC, Chen TW, Kim DS, Garaschuk O, Griesinger C, Griesbeck O.
Nat Methods. 2014 Jan 5. doi: 10.1038/nmeth.2773. [Epub ahead of print]

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2013

Structural model for the covalent adhesion of the Streptococcus pyogenes pilus through a thioester bond.
Linke-Winnebeck C, Paterson NG, Young PG, Middleditch MJ, Greenwood DR, Witte G, Baker EN.
J Biol Chem. 2013 Nov 12. [Epub ahead of print]

cGAS produces a 2'-5'-linked cyclic dinucleotide second messenger that activates STING.
Ablasser A, Goldeck M, Cavlar T, Deimling T, Witte G, Röhl I, Hopfner KP, Ludwig J, Hornung V.
Nature. 2013 May 30. doi: 10.1038/nature12306

Structural mechanism of cytosolic DNA sensing by cGAS.
Civril F, Deimling T, de Oliveira Mann CC, Ablasser A, Moldt M, Witte G, Hornung V, Hopfner KP
Nature. 2013 May 30. doi: 10.1038/nature12305

Paramyxovirus V Proteins Disrupt the Fold of the RNA Sensor MDA5 to Inhibit Antiviral Signaling
Motz C, Schuhmann KM, Kirchhofer A, Moldt M, Witte G, Conzelmann KK, Hopfner KP
Science January 17 2013 Science DOI: 10.1126/science.1230949

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2012

Structural characterization of a eukaryotic chaperone—the ribosome-associated complex
Leidig C,Bange G, Kopp J, Amlacher S, Aravind A, Wickles S, Witte G, Hurt E, Beckmann R, Sinning I
NSMB 02 December 2012 | doi:10.1038/nsmb.2447

Structure of Actin-related protein 8 and its contribution to nucleosome binding.
Gerhold CB, Winkler DD, Lakomek K, Seifert FU, Fenn S, Kessler B, Witte G, Luger K, Hopfner KP.
Nucleic Acids Res. 2012 Sep 12. [Epub ahead of print]

Correlating calcium binding, Förster resonance energy transfer, and conformational change in the biosensor TN-XXL.
Geiger A, Russo L, Gensch T, Thestrup T, Becker S, Hopfner KP, Griesinger C, Witte G, Griesbeck O.
Biophys J. 2012 May 16;102(10):2401-10. Epub 2012 May 15.

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2011

The RIG-I ATPase domain structure reveals insights into ATP-dependent antiviral signalling.
Civril F, Bennett M, Moldt M, Deimling T, Witte G, Schiesser S, Carell T, Hopfner KP
EMBO reports 2011 Oct 28;12(11):1127-34. doi: 10.1038/embor.2011.190.

Characterization of the chi psi subcomplex of Pseudomonas aeruginosa DNA polymerase III.
El Houry Mignan S and Witte G, Naue N, Curth U.
BMC Mol Biol. 2011 Sep 28;12(1):43.

Molecular basis of Rrn3-regulated RNA polymerase I initiation and cell growth.
Blattner C, Jennebach S, Herzog F, Mayer A, Cheung AC, Witte G, Lorenzen K, Hopfner KP, Heck AJ, Aebersold R, Cramer P.
Genes Dev. 2011 Sep 22. Oct 1;25(19):2093-105

Structure and mechanism of the Swi2/Snf2 remodeller Mot1 in complex with its substrate TBP.
Wollmann P, Cui S, Viswanathan R, Berninghausen O, Wells MN, Moldt M, Witte G, Butryn A, Wendler P, Beckmann R, Auble DT, Hopfner KP.
Nature. 2011 Jul 6;475(7356):403-7. doi: 10.1038/nature10215.

High-resoltion X-ray structure of the trimeric Scar/WAVE-complex precursor Brk1
Linkner J, Witte G*, Stradal T, Curth U, Faix J
PLoS One. 2011;6(6):e21327. Epub 2011 Jun 20.

Structural biochemistry of nuclear actin-related proteins 4 and 8 reveals their interaction with actin.
Fenn S, Breitsprecher D, Gerhold CB, Witte G, Faix J, Hopfner KP.
EMBO J. 2011 Jun 1;30(11):2153-66. Epub 2011 Apr 15.

The phenotypic spectrum of neutral lipid storage myopathy due to mutations in the PNPLA2 gene.
Reilich P, Horvath R, Krause S, Schramm N, Turnbull DM, Trenell M, Hollingsworth KG, Gorman GS, Hans VH, Reimann J, Macmillan A, Turner L, Schollen A, Witte G, Czermin B, Holinski-Feder E, Walter MC, Schoser B, Lochmüller H.
J Neurol. 2011 May 5.

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2010

Structure and DNA binding activity of the mouse condensin hinge domain highlight common and diverse features of SMC proteins.
Griese JJ, Witte G, Hopfner KP.
Nucleic Acids Res. 2010 Jun;38(10):3454-65. Epub 2010 Feb 5.

Structural biochemistry of a bacterial checkpoint protein reveals diadenylate cyclase activity regulated by DNA recombination intermediates.
Witte G, Hartung S, Büttner K, Hopfner KP.
Mol Cell. 2008 Apr 25;30(2):167-78.
Faculty of 1000 rating 10
Editor's choice in Science Signaling (Sci. Signal., 29 April 2008 Vol. 1, Issue 17, p. ec151)
Perspective in Science Signaling by Ute Römling (Sci. Signal., 19 August 2008 Vol. 1, Issue 33, p. pe39)
Chosen as ESRF Scientific Highlight 2008 (ESRF Highlights 2008, p56)

Biophysical analysis of Thermus aquaticus single-stranded DNA binding protein.
Witte G, Fedorov R, Curth U.
Biophys J. 2008 Mar 15;94(6):2269-79. Epub 2007 Dec 7.

3D structure of Thermus aquaticus single-stranded DNA-binding protein gives insight into the functioning of SSB proteins.
Fedorov R, Witte G, Urbanke C, Manstein DJ, Curth U.
Nucleic Acids Res. 2006 ;34(22):6708-17.

Single-stranded DNA-binding protein of Deinococcus radiodurans: a biophysical characterization.
Witte G, Urbanke C, Curth U.
Nucleic Acids Res. 2005 Mar 21;33(5):1662-70.

A Sedimentation velocity method in the analytical ultracentrifuge for the study of protein-protein interactions.
Urbanke C, Witte G, Curth U.
Methods Mol Biol. 2005;305:101-14. PubMed PMID: 15939995.

DNA polymerase III chi subunit ties single-stranded DNA binding protein to the bacterial replication machinery.
Witte G, Urbanke C, Curth U.
Nucleic Acids Res. 2003 Aug 1;31(15):4434-40.

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